Molecular Properties

Molecular properties

This sub-section evaluates the usage of gene expression data to classify and model single neurons.

The frequency of expression of commonly expressed calcium binding proteins and neuropeptides obtained from RT-PCR experiments were evaluated in the reconstructed microcircuit.

The results of the consistency check were categorized as consistent, inconsistent, possibly consistent, not implemented or not applicable based on agreement with a given evaluation criterion.

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consistent - Nathaniel Heintz - Application of a translational profiling approach for the comparative analysis of CNS cell types.

consistent - Burkhalter, A - Axo-axonic synapses formed by somatostatin-expressing GABAergic neurons in rat and monkey visual cortex.

consistent - Cauli, B - Classification of NPY-expressing neocortical interneurons.

input/validation/consistent: consistent
evaluation criterion: The reporetd gene-types were used to validate the m-type composition of the reconstructed microcircuit
id: 3
should be in: mol
feedback: follow if time
species: rat
age: P17-21
brain region: barrel cortex
layer: each
cell: interneurons NPY expressing
aspect: morphology, neurochemical, electrical
statement: To classify reliably cortical NPY neurons, used unsupervised clustering algorithms based on laminar location and electrophysiological and molecular properties. These classification schemes confirmed that NPY neurons are nearly exclusively GABAergic and are three main types (1) Neurogliaform-like neurons exhibiting a dense axonal arbor, were the most frequent and superficial, and substantially expressed the neuronal isoform of nitric oxide synthase. (2) Martinotti-like cells characterized by an ascending axon ramifying in layer I coexpressed somatostatin and were the most excitable type. (3) Among fast-spiking and parvalbumin-positive basket cells, NPY expression was correlated with pronounced spike latency.
pmid: 19295167
title: Classification of NPY-expressing neocortical interneurons.
contact Name: Cauli, B
contributed by: Monica Favre
checked by: DK

Digital Reconstruction of Neocortical Microcircuitry

Molecular properties

consistent - Nathaniel Heintz - Application of a translational profiling approach for the comparative analysis of CNS cell types.

consistent - Burkhalter, A - Axo-axonic synapses formed by somatostatin-expressing GABAergic neurons in rat and monkey visual cortex.

consistent - Cauli, B - Classification of NPY-expressing neocortical interneurons.

consistent - Andreas Bosio - Gene expression analysis defines differences between region-specific GABAergic neurons.

consistent - Sacha B Nelson - Molecular taxonomy of major neuronal classes in the adult mouse forebrain.

consistent - Yasuo Kawaguchi - Selective coexpression of multiple chemical markers defines discrete populations of neocortical GABAergic neurons.